Multiplexed protein imaging
COMET sequential immunofluorescence analysis
Automated sequential immunofluorescence, analyzed into clean phenotypes and spatial results.
What COMET measures
COMET automates sequential immunofluorescence: rounds of staining, imaging and gentle elution on a microfluidic chip build up multiplex panels of up to around 40 markers on a single section, using standard off-the-shelf antibodies.
Strengths
- Hands-off automated staining and imaging
- Uses standard, unconjugated primary antibodies
- Up to roughly 40 markers on one section
- Fast turnaround from protocol to multiplex data
How we can help with COMET data
Image QC and background
Checking cycles and correcting background and autofluorescence.
Segmentation
Deep learning cell segmentation from nuclear and membrane markers.
Phenotyping
Defining cell types by thresholds, clustering or both.
Spatial analysis
Neighbourhoods, proximity and region comparisons.
QuPath workflows
Open, reproducible projects and scripts.
Figures
Composite images and statistics for publication.
Related technologies
Working with COMET data?
Tell us about your samples, panel and question. The first consultation is free.