Multiplexed protein imaging
PhenoImager and Opal multiplex IF analysis
Quantitative multiplex immunofluorescence for translational studies and clinical-trial endpoints.
What PhenoImager & Opal measures
PhenoImager systems, formerly Vectra Polaris, image multiplex immunofluorescence stained with Opal tyramide signal amplification. Panels of around 6 to 9 markers are separated by multispectral unmixing, giving quantitative whole-slide data that is widely used in translational research and clinical trials.
Strengths
- Robust, quantitative multiplex IF suited to clinical cohorts
- Multispectral unmixing reduces autofluorescence and spillover
- Whole-slide scanning
- Established workflows in translational and trial settings
How we can help with PhenoImager & Opal data
Unmixed image analysis
Working from unmixed component images or inForm and HALO exports.
Segmentation and phenotyping
StarDist cell detection and marker-based classification in QuPath.
Tumour microenvironment
Immune infiltration, tumour–immune proximity and density metrics.
Trial endpoints
Reproducible quantification suited to clinical-trial and cohort endpoints.
Batch correction
Normalization across slides, runs and cohorts.
Open-source migration
Re-creating proprietary analyses in open tools you control.
Related technologies
Working with PhenoImager & Opal data?
Tell us about your samples, panel and question. The first consultation is free.